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Product Info Summary
| SKU: | EK0935 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse GDNF ELISA Kit PicoKine®
SKU/Catalog Number
EK0935
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse GDNF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Gdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse GDNF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0935)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard:NS0; Immunogen sequence: S78-I211
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0935 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0935 is reactive to GDNF in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0935 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GDNF
Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0935-CAP | Anti-Mouse GDNF Pre-coated 96-well strip microplate | 1 |
| EK0935-ST | Mouse GDNF Standard | 2 vials, 10 ng/tube |
| EK0935-DA | Mouse GDNF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.047 | 0.069 | 0.090 | 0.164 | 0.312 | 0.577 | 1.014 | 1.808 |
Data Example Images
Click image to see more details
Mouse GDNF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GDNF, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Some articles we found to cite concentrations of GDNF in samples: 14521734 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse GDNF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 101 | 227 | 789 | 101 | 217 | 855 |
| Standard deviation | 7.57 | 9.3 | 57.53 | 7.67 | 9.11 | 66.69 |
| CV (%) | 7.5% | 4.1% | 7% | 7.6% | 4.2% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GDNF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 101 | 101 | 104 | 97 | 100 | 2.48 | 2.4% |
| Sample 2 | 227 | 204 | 214 | 227 | 218 | 9.66 | 4.4% |
| Sample 3 | 789 | 928 | 792 | 891 | 850 | 60.93 | 7.1% |
Protein Target Info & Infographic
Gene/Protein Information For GDNF (Source: Uniprot.Org, NCBI)
Gene Name
GDNF
Full Name
Glial cell line-derived neurotrophic factor
Weight
23.72kDa
Superfamily
TGF-beta family
Alternative Names
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3 GDNF ATF, ATF1, ATF2, HFB1-GDNF, HSCR3 glial cell derived neurotrophic factor glial cell line-derived neurotrophic factor|astrocyte-derived trophic factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GDNF, check out the GDNF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GDNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse GDNF ELISA Kit PicoKine® (EK0935)
Hello CJ!
EK0935 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Granulocyte-colony stimulating factor promotes brain repair following traumatic brain injury by recruitment of microglia and increasing neurotrophic factor expression
Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells
Neurogenesis within the hippocampus after chronic methylphenidate exposure
Granulocyte colony‐stimulating factor promotes behavioral recovery in a mouse model of traumatic brain injury
Granulocyte colony-stimulating factor promotes behavioral recovery in a mouse model of traumatic brain injury
Yang C, Zhou L, Gao X, Chen B, Tu J, Sun H, Liu X, He J, Liu J, Yuan Q. Neurosurgery. 2011 Mar;68(3):691-704. Doi: 10.1227/Neu.0B013E3182098A8A. Neuroprotective Effects Of Bone Marrow Stem Cells Overexpressing Glial Cell Line-Derived Neurotrophic ...
Liu G, Wang X, Shao G, Liu Q. Mol Med Rep. 2014 Apr;9(4):1305-12. Doi: 10.3892/Mmr.2014.1963. Epub 2014 Feb 18. Genetically Modified Schwann Cells Producing Glial Cell Line-Derived Neurotrophic Factor Inhibit Neuronal Apoptosis In Rat Spinal Cord ...
Chai L, Guo H, Li H, Wang S, Wang Yl, Shi F, Hu Lm, Liu Y, Adah D. J Ethnopharmacol. 2013 Oct 28;150(1):100-7. Doi: 10.1016/J.Jep.2013.08.011. Epub 2013 Sep 4. Scutellarin And Caffeic Acid Ester Fraction, Active Components Of Dengzhanxixin Injecti...
Banerjee A, N??rnberger S, Hennerbichler S, Riedl S, Schuh Cm, Hacobian A, Teuschl A, Eibl J, Redl H, Wolbank S. Cell Tissue Bank. 2014 Jun;15(2):227-39. Doi: 10.1007/S10561-013-9401-1. Epub 2013 Oct 29. In Toto Differentiation Of Human Amniotic M...
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Customer Reviews
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1 Reviews For Mouse GDNF ELISA Kit PicoKine®
0
Easy to Use GDNF ELISA Kit--Hannah Oakes, EAST TENNESSEE STATE UNIVERSITY, BIOMEDICAL SCIENCES, GRADUATE ASSISTANT
Excellent
Source: Biocompare.com
| Applications | Detecting GDNF |
|---|---|
| Sample | Brain Tissue (Hippocampus) |
| Detection | -- |
"We used this kit and the BDNF kit, because we were looking at neurogenesis within the hippocampus. We used the ELISA to complement our immunohistochemistry. It was easy to optimize the kits and the instructions were extremely easy to follow. Additionally, the instructions do not include using samples from tissue homogenates, but we did and the plates came out great. This product was selected after speaking with a representative about it at a conference. I am extremely glad we decided to go with this kit. This is a great kit. The protocol was easy to follow and optimize. We will be using these kits again."
Hannah Oakes
Verified customer
Submitted 2016-01-21
Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Mouse GDNF ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-14
Question
Would EK0935 work on tissue homogenate samples that has been prepared with RIPA buffer?
Verified customer
Asked: 2019-04-01
Answer
Mouse GDNF ELISA Kit PicoKine™ (EK0935) hasn't been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend to run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-04-02
Question
Q: if the enzyme conjugated GDNF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GDNF antigen?
Verified Customer
Verified customer
Asked: 2018-05-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-05-21
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-03-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-03-30
Question
Q: how do I analyze ELISA data? I have performed GDNF level in plasma.
Verified Customer
Verified customer
Asked: 2015-03-27
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-03-27
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis GDNF using the EK0935 Mouse GDNF Picokine® ELISA Kit?
E. Liu
Verified customer
Asked: 2014-11-24
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2014-11-24
